Feedback Inhibition of Purine Biosynthesis in Ascites Tumor Cells

Feedback Inhibition of Purine Biosynthesis in Ascites Tumor Cells*

Feedback inhibition of purine biosynthesis de novo in bacteria has been studied in some detail by Gots and his colleagues (l-4). Other observations have suggested feedback inhibition, but these are complicated by dilution of intermediary pools by the added inhibitor (5-10). This reaction has been studied less extensively in mammalian cells. Sartorelli and LePage (11) and LePage and Jones (12) h...

Feedback inhibition of purine biosynthesis in ascites tumor cells by purine analogues.

Feedback inhibition of purine biosynthesis de novo in bacteria has been studied in some detail by Gots and his colleagues (l-4). Other observations have suggested feedback inhibition, but these are complicated by dilution of intermediary pools by the added inhibitor (5-10). This reaction has been studied less extensively in mammalian cells. Sartorelli and LePage (11) and LePage and Jones (12) h...

On the Mechanism of Feedback Inhibition of Purine Biosynthesis De Novo in Ehrlich Ascites Tumor Cells in Vitro.

Feedback inhibition of biosynthetic pathways in animal cells may be investigated at three levels of complexity. (a) Enzymological studies may evaluate the relative sensitivity of various enzymes of the pathway to inhibition by end products, the relative inhibitory potencies of different end products, and the mode of interaction of inhibitors and enzymes. In the absence of other information, how...

Purinethiols as feedback inhibitors of purine synthesis in ascites tumor cells.

The capacity of several purinethiol derivatives to produce prolongation of survival in mice bearing ascites-cell tumors and to produce feedback inhibition of purine synthesis in vivo in the tumor cells was investigated. Included were tumors susceptible and tumors resistant to treatment with 6-thioguanine. With susceptible tumors, certain of the purinethiols could increase survival of tumor-bear...

Pharmacologic regulation of the early steps of purine biosynthesis in Burkitt lymphoma cells and in Ehrlich ascites tumor cells.